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cell lysis solution (wizard genomic dna purification kit)  (Promega)

 
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    Structured Review

    Promega cell lysis solution (wizard genomic dna purification kit)
    Cell Lysis Solution (Wizard Genomic Dna Purification Kit), supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+lysis+solution+wizard+genomic+dna+purification+kit/cell+lysis+solution/pm40301684-237-27-32
    Average 90 stars, based on 1 article reviews
    cell lysis solution (wizard genomic dna purification kit) - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Lysis:

    Article Title: Extracellular vesicles of minimalistic Mollicutes as mediators of immune modulation and horizontal gene transfer
    Article Snippet: .. To screen for transformants obtained by vesiduction, colonies were picked in 1 ml selective SP5 and lysed using cell lysis solution (Wizard Genomic DNA Purification Kit, Promega). .. 1 μl of lysed cells was used as template in a PCR using the tet -specific primers for the plasmid pIVB08 and strain-specific primers (Table ) in a 10 μl reaction in GoTaq G2 Green Master Mix (Promega) according to manufacturer’s instructions and PCR products were visualized on a 2% agarose in TAE-buffer gel with RedSafe (iNtRON Biotechnology).

    Article Title: Extracellular vesicles of minimalistic Mollicutes as mediators of immune modulation and horizontal gene transfer
    Article Snippet: The DNase treated EV sample was then resuspended in 12 ml PBS, pelleted as described above and finally lysed by resuspending it in 50 μl cell lysis solution (Wizard Genomic DNA Purification Kit, Promega). .. Bacterial cells of 8 ml culture in SP5 were pelleted, washed in PBS and resuspended in 500 μl cell lysis solution (Wizard Genomic DNA Purification Kit, Promega). qPCR was conducted in a 10 μl reaction volume with template DNA equalized to 600 ng (EV lysate or bacterial cell lysate) and primers detailed in Table . .. SsoFast EvaGreen Supermix (Biorad) was used according to manufacturer’s instruction and the reaction was run for 40 cycles in a QuantStudioTM 5 System machine (ThermoFisher Scientific).

    Article Title: Extracellular vesicles of minimalistic Mollicutes as mediators of immune modulation and horizontal gene transfer
    Article Snippet: First, extravesicular DNA was removed by adding 1 μl of DNase I at 10 U/μl (Roche) per ml of the EV sample and incubated at 37 °C for 30 min, before the DNase was inactivated at 75 °C for 10 min. .. The DNase treated EV sample was then resuspended in 12 ml PBS, pelleted as described above and finally lysed by resuspending it in 50 μl cell lysis solution (Wizard Genomic DNA Purification Kit, Promega). .. Bacterial cells of 8 ml culture in SP5 were pelleted, washed in PBS and resuspended in 500 μl cell lysis solution (Wizard Genomic DNA Purification Kit, Promega). qPCR was conducted in a 10 μl reaction volume with template DNA equalized to 600 ng (EV lysate or bacterial cell lysate) and primers detailed in Table .

    DNA Purification:

    Article Title: Extracellular vesicles of minimalistic Mollicutes as mediators of immune modulation and horizontal gene transfer
    Article Snippet: .. To screen for transformants obtained by vesiduction, colonies were picked in 1 ml selective SP5 and lysed using cell lysis solution (Wizard Genomic DNA Purification Kit, Promega). .. 1 μl of lysed cells was used as template in a PCR using the tet -specific primers for the plasmid pIVB08 and strain-specific primers (Table ) in a 10 μl reaction in GoTaq G2 Green Master Mix (Promega) according to manufacturer’s instructions and PCR products were visualized on a 2% agarose in TAE-buffer gel with RedSafe (iNtRON Biotechnology).

    Article Title: Extracellular vesicles of minimalistic Mollicutes as mediators of immune modulation and horizontal gene transfer
    Article Snippet: The DNase treated EV sample was then resuspended in 12 ml PBS, pelleted as described above and finally lysed by resuspending it in 50 μl cell lysis solution (Wizard Genomic DNA Purification Kit, Promega). .. Bacterial cells of 8 ml culture in SP5 were pelleted, washed in PBS and resuspended in 500 μl cell lysis solution (Wizard Genomic DNA Purification Kit, Promega). qPCR was conducted in a 10 μl reaction volume with template DNA equalized to 600 ng (EV lysate or bacterial cell lysate) and primers detailed in Table . .. SsoFast EvaGreen Supermix (Biorad) was used according to manufacturer’s instruction and the reaction was run for 40 cycles in a QuantStudioTM 5 System machine (ThermoFisher Scientific).

    Article Title: Extracellular vesicles of minimalistic Mollicutes as mediators of immune modulation and horizontal gene transfer
    Article Snippet: First, extravesicular DNA was removed by adding 1 μl of DNase I at 10 U/μl (Roche) per ml of the EV sample and incubated at 37 °C for 30 min, before the DNase was inactivated at 75 °C for 10 min. .. The DNase treated EV sample was then resuspended in 12 ml PBS, pelleted as described above and finally lysed by resuspending it in 50 μl cell lysis solution (Wizard Genomic DNA Purification Kit, Promega). .. Bacterial cells of 8 ml culture in SP5 were pelleted, washed in PBS and resuspended in 500 μl cell lysis solution (Wizard Genomic DNA Purification Kit, Promega). qPCR was conducted in a 10 μl reaction volume with template DNA equalized to 600 ng (EV lysate or bacterial cell lysate) and primers detailed in Table .

    Real-time Polymerase Chain Reaction:

    Article Title: Extracellular vesicles of minimalistic Mollicutes as mediators of immune modulation and horizontal gene transfer
    Article Snippet: The DNase treated EV sample was then resuspended in 12 ml PBS, pelleted as described above and finally lysed by resuspending it in 50 μl cell lysis solution (Wizard Genomic DNA Purification Kit, Promega). .. Bacterial cells of 8 ml culture in SP5 were pelleted, washed in PBS and resuspended in 500 μl cell lysis solution (Wizard Genomic DNA Purification Kit, Promega). qPCR was conducted in a 10 μl reaction volume with template DNA equalized to 600 ng (EV lysate or bacterial cell lysate) and primers detailed in Table . .. SsoFast EvaGreen Supermix (Biorad) was used according to manufacturer’s instruction and the reaction was run for 40 cycles in a QuantStudioTM 5 System machine (ThermoFisher Scientific).



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